OS9 Antikörper (AA 56-635)

Produktnummer ABIN7602048

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch OS9 in WB, ELISA, ICC und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für OS9 Antikörper (AA 56-635) (ABIN7602048)

Target: OS9 (Osteosarcoma Amplified 9, Endoplasmic Reticulum Lectin (OS9))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OS9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-OS9 Antikörper

Bindungsspezifität: AA 56-635

Verwendungszweck: Anti-OS9 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-OS9 Antibody Picoband® (ABIN7602048). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human OS9 recombinant protein (Position: Q56-E635).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baek, J. H., Mahon, P. C., Oh, J., Kelly, B., Krishnamachary, B., Pearson, M., Chan, D. A., Giaccia, A. J., Semenza, G. L. OS-9 interacts with hypoxia-inducible factor 1-alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1-alpha. Molec. Cell 17: 503-512, 2005. 2. Bhamidipati, A., Denic, V., Quan, E. M., Weissman, J. S. Exploration of the topological requirements of ERAD identifies Yos9p as a lectin sensor of misfolded glycoproteins in the ER lumen. Molec. Cell 19: 741-751, 2005. 3. Friedmann, E., Salzberg, Y., Weinberger, A., Shaltiel, S., Gerst, J. E. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins. J. Biol. Chem. 277: 35274-35281, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu OS9

Target: OS9 (Osteosarcoma Amplified 9, Endoplasmic Reticulum Lectin (OS9))

Andere Bezeichnung: OS9

Hintergrund:

Synonyms: Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Erf1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: Protein OS-9 is a protein that in humans is encoded by the OS9 gene. This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molekulargewicht: 83 kDa

Gen-ID: 10956

UniProt: Q13438

Pathways: Maintenance of Protein Location, SARS-CoV-2 Protein Interaktom