IWS1 Antikörper (AA 547-771)

Produktnummer ABIN7602008

Produktdetails anti-IWS1 Antikörper

Target: IWS1 (IWS1 Homolog (IWS1))

Bindungsspezifität: AA 547-771

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IWS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Verwendungszweck: Anti-IWS1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-IWS1 Antibody Picoband® (ABIN7602008). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human IWS1 recombinant protein (Position: R547-E771).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Yoh, S. M. , Cho, H. , Pickle, L. , Evans, R. M. , & Jones, K. A. . (2007). The spt6 sh2 domain binds ser2-p rnapii to iws1-dependent mrna splicing and export. Genes & Development, 21(2), 160. 2. Yoh, S. M. , Lucas, J. S. , & Jones, K. A. . (2008). The iws1:spt6:ctd complex controls cotranscriptional mrna biosynthesis and hypb/setd2-mediated histone h3k36 methylation. Genes Dev, 22(24), 3422-3434.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu IWS1

Target: IWS1 (IWS1 Homolog (IWS1))

Andere Bezeichnung: IWS1 (IWS1 Produkte)

Hintergrund:

Synonyms: Long-chain-fatty-acid--CoA ligase 3,6.2.1.3,Long-chain acyl-CoA synthetase 3,LACS 3,ACSL3,ACS3, FACL3, LACS3,

Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Background: Protein IWS1 homolog also known as interacts with Spt6 (IWS1) is a protein that in humans is encoded by the IWS1 gene. Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps. IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone. IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation. Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level .

Molekulargewicht: 140 kDa

Gen-ID: 55677