Nth Endonuclease III-Like 1 (NTHL1) (AA 52-312) Antikörper

Produktnummer ABIN7601953

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB, ELISA, IF, ICC und FACS validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Nth Endonuclease III-Like 1 (NTHL1) (AA 52-312) Antikörper (ABIN7601953)

Target: Nth Endonuclease III-Like 1 (NTHL1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails

Bindungsspezifität: AA 52-312

Verwendungszweck: Anti-NTHL1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NTHL1 Antibody Picoband® (ABIN7601953). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NTHL1 recombinant protein (Position: H52-L312).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aspinwall, R., Rothwell, D. G., Roldan-Arjona, T., Anselmino, C., Ward, C. J., Cheadle, J. P., Sampson, J. R., Lindahl, T., Harris, P. C., Hickson, I. D. Cloning and characterization of a functional human homolog of Escherichia coli endonuclease III. Proc. Nat. Acad. Sci. 94: 109-114, 1997. 2. Hilbert, T. P., Chaung, W., Boorstein, R. J., Cunningham, R. P., Teebor, G. W. Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, Escherichia coli endonuclease III. J. Biol. Chem. 272: 6733-6740, 1997. 3. Imai, K., Sarker, A. H., Akiyama, K., Ikeda, S., Yao, M., Tsutsui, K., Shohmori, T., Seki, S. Genomic structure and sequence of a human homologue (NTHL1/NTH1) of Escherichia coli endonuclease III with those of the adjacent parts of TSC2 and SLC9A3R2 genes. Gene 222: 287-295, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Antigendetails

Target: Nth Endonuclease III-Like 1 (NTHL1)

Andere Bezeichnung: NTHL1

Hintergrund:

Synonyms: Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR, KIAA1999

Tissue Specificity: In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.

Background: Endonuclease III-like protein 1 is an enzyme that in humans is encoded by the NTHL1 gene. The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.

Molekulargewicht: 34 kDa

Gen-ID: 4913

UniProt: P78549