COMT Antikörper (AA 52-271)

Produktnummer ABIN7601948

Dieses Anti-COMT-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von COMT in WB, IHC, ICC, IF und FACS. Geeignet für Human, Ratte und Maus.

Kurzübersicht für COMT Antikörper (AA 52-271) (ABIN7601948)

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 15C10

Produktdetails anti-COMT Antikörper

Bindungsspezifität: AA 52-271

Verwendungszweck: Anti-COMT Antibody Picoband® (monoclonal, 15C10)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-COMT Antibody Picoband® (monoclonal, 15C10) (ABIN7601948). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human COMT recombinant protein (Position: G52-P271). Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Baker, K., Baldeweg, T., Sivagnanasundaram, S., Scambler, P., Skuse, D. COMT val108/158met modifies mismatch negativity and cognitive function in 22q11 deletion syndrome. Biol. Psychiat. 58: 23-31, 2005. 2. Lee, S.-G., Joo, Y., Kim, B., Chung, S., Kim, H.-L., Lee, I., Choi, B., Kim, C., Song, K.Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans. Hum. Genet. 116: 319-328, 2005.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Andere Bezeichnung: COMT

Hintergrund:

Synonyms: Catechol O-methyltransferase, COMT

Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.

Background: Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.

Molekulargewicht: 29 kDa

Gen-ID: 1312

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interaktom