PDE10A Antikörper (AA 515-779)

Produktnummer ABIN7601943

Produktdetails anti-PDE10A Antikörper

Target: PDE10A (phosphodiesterase 10A (PDE10A))

Bindungsspezifität: AA 515-779

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDE10A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Verwendungszweck: Anti-PDE10A Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PDE10A Antibody Picoband® (ABIN7601943). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PDE10A recombinant protein (Position: H515-D779).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Diggle, C. P., Sukoff Rizzo, S. J., Popiolek, M., Hinttala, R., Schulke J.-P., Kurian, M. A., Carr, I. M., Markham, A. F., Bonthron, D. T., Watson, C., Sharif, S. M., Reinhart, V., and 30 others. Biallelic mutations in PDE10A lead to loss of striatal PDE10A and a hyperkinetic movement disorder with onset in infancy. Am. J. Hum. Genet. 98: 735-743, 2016. 2. Fujishige, K., Kotera, J., Michibata, H., Yuasa, K., Takebayashi, S., Okumura, K., Omori, K. Cloning and characterization of a novel human phosphodiesterase that hydrolyzes both cAMP and cGMP (PDE10A). J. Biol. Chem. 274: 18438-18445, 1999. 3. Fujishige, K., Kotera, J., Yuasa, K., Omori, K. The human phosphodiesterase PDE10A gene: genomic organization and evolutionarily relatedness with other PDEs containing GAF domains. Europ. J. Biochem. 267: 5943-5951, 2000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PDE10A

Target: PDE10A (phosphodiesterase 10A (PDE10A))

Andere Bezeichnung: PDE10A (PDE10A Produkte)

Hintergrund:

Synonyms: Homeobox protein SIX3,Sine oculis homeobox homolog 3,SIX3,

Tissue Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.

Background: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene.

Molekulargewicht: 100 kDa

Gen-ID: 10846

UniProt: Q9Y233

Pathways: cAMP Metabolic Process