ECD/SGT1 Antikörper (AA 50-632)

Produktnummer ABIN7601917

Der Kaninchen Polyklonal Anti-ECD/SGT1-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, ECD/SGT1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für ECD/SGT1 Antikörper (AA 50-632) (ABIN7601917)

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ECD/SGT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-ECD/SGT1 Antikörper

Bindungsspezifität: AA 50-632

Verwendungszweck: Anti-SGT1/ECD Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SGT1/ECD Antibody Picoband® (ABIN7601917). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ECD recombinant protein (Position: L50-N632).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 7/9/2015. 2. Kim, J. H., Gurumurthy, C. B., Band, H., Band, V. Biochemical characterization of human Ecdysoneless reveals a role in transcriptional regulation. Biol. Chem. 391: 9-19, 2010. 3. Sato, T., Jigami, Y., Suzuki, T., Uemura, H. A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae. Molec. Gen. Genet. 260: 535-540, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ECD/SGT1

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Andere Bezeichnung: ECD

Hintergrund:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, CD156c, ADAM10, KUZ, MADM

Tissue Specificity: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Background: Protein SGT1 is a protein that in humans is encoded by the ECD gene. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Molekulargewicht: 73 kDa

Gen-ID: 11319

UniProt: O95905

Pathways: Regulation of Carbohydrate Metabolic Process