BUB3 Antikörper (AA 50-209)

Produktnummer ABIN7601900

Der Kaninchen Polyklonal anti-BUB3 Antikörper (ABIN7601900) detektiert spezifisch BUB3 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für BUB3 Antikörper (AA 50-209) (ABIN7601900)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BUB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-BUB3 Antikörper

Bindungsspezifität: AA 50-209

Verwendungszweck: Anti-BUB3 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BUB3 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BUB3 recombinant protein (Position: R50-D209).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Andere Bezeichnung: BUB3

Hintergrund:

Background: BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC), though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through ion of kinetochore localization during prometaphase to achieve biorentation. Bub3 s the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.

Gene Full Name: BUB3 mitotic checkpoint protein

Molekulargewicht: 40 kDa

Gen-ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location