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GOT1 Antikörper (AA 5-413)

Der Kaninchen Polyklonal Anti-GOT1-Antikörper wurde für WB, IHC, ELISA, ICC, IF und FACS validiert. Er ist geeignet, GOT1 in Proben von Human, Maus und Ratte zu detektieren.
Produktnummer ABIN7601884

Kurzübersicht für GOT1 Antikörper (AA 5-413) (ABIN7601884)

Target

Alle GOT1 Antikörper anzeigen
GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reaktivität

  • 75
  • 39
  • 24
  • 7
  • 7
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 79
  • 20
  • 4
  • 3
  • 3
Kaninchen

Klonalität

  • 87
  • 22
Polyklonal

Konjugat

  • 62
  • 18
  • 9
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Dieser GOT1 Antikörper ist unkonjugiert

Applikation

  • 92
  • 59
  • 30
  • 25
  • 23
  • 14
  • 13
  • 10
  • 9
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 15
    • 10
    • 9
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 5-413

    Verwendungszweck

    Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (ABIN7601884). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human Aspartate Aminotransferase/GOT1 recombinant protein (Position: S5-Q413).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Aitken, D. A., Ferguson-Smith, M. A. Gene dosage evidence for the regional assignment of the GOT-S structural gene locus to 10q24-10q25. Cytogenet. Cell Genet. 22: 468-471, 1978. 2. Creagan, R., Tischfield, J., McMorris, F. A., Chen, S.-H., Hirschi, M., Chen, T.-T., Ricciuti, F., Ruddle, F. H. Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic-cell hybrids. Cytogenet. Cell Genet. 12: 187-198, 1973. 3. Doonan, S., Barra, D., Bossa, F. Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int. J. Biochem. 16: 1193-1199, 1984.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

    Andere Bezeichnung

    GOT1

    Hintergrund

    Synonyms: Muscarinic acetylcholine receptor M1, CHRM1

    Tissue Specificity: Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

    Background: Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

    Molekulargewicht

    41 kDa

    Gen-ID

    2805

    UniProt

    P17174

    Pathways

    Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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