AFAP Antikörper (AA 498-730)

Produktnummer ABIN7601861

Dieses Anti-AFAP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von AFAP in WB, ELISA, IHC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für AFAP Antikörper (AA 498-730) (ABIN7601861)

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-AFAP Antikörper

Bindungsspezifität: AA 498-730

Verwendungszweck: Anti-AFAP/AFAP1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-AFAP/AFAP1 Antibody Picoband® (ABIN7601861). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human AFAP/AFAP1 recombinant protein (Position: A498-T730).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL,Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baisden, J. M., Qian, Y., Zot, H. M., Flynn, D. C. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity. Oncogene 20: 6435-6447, 2001. 2. Flynn, D. C., Leu, T. H., Reynolds, A. B., Parsons, J. T. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Molec. Cell Biol. 13: 7892-7900, 1993. 3. Kanner, S. B., Reynolds, A. B., Wang, H.-C. R., Vines, R. R., Parsons, J. T. The SH2 and SH3 domains of pp60src stable association with tyrosine phosphorylated proteins p130 and p110. EMBO J. 10: 1689-1698, 1991.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu AFAP

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Andere Bezeichnung: AFAP1

Hintergrund:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: Actin filament-associated protein 1 is a protein that in humans is encoded by the AFAP1 gene. The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 120 kDa

Gen-ID: 60312

UniProt: Q8N556