ChT Antikörper (AA 446-580)

Produktnummer ABIN7601754

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ChT in WB, FACS und ELISA. Er zeigt eine Reaktivität gegenüber Maus, Human und Ratte.

Kurzübersicht für ChT Antikörper (AA 446-580) (ABIN7601754)

Target: ChT (High Affinity Choline Transporter (ChT))

Reaktivität: Maus, Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ChT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Produktdetails anti-ChT Antikörper

Bindungsspezifität: AA 446-580

Verwendungszweck: Anti-SLC5A7 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SLC5A7 Antibody Picoband® (ABIN7601754). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Apparsundaram, S., Ferguson, S. M., George, A. L., Jr., Blakely, R. D. Molecular cloning of a human, hemicholinium-3-sensitive choline transporter. Biochem. Biophys. Res. Commun. 276: 862-867, 2000. 2. Barwick, K. E. S., Wright, J., Al-Turki, S., McEntagart, M. M., Nair, A., Chioza, B., Al-Memar, A., Modarres, H., Reilly, M. M., Dick, K. J., Ruggiero, A. M., Blakely, R. D., Hurles, M. E., Crosby, A. H. Defective presynaptic choline transport underlies hereditary motor neuropathy. Am. J. Hum. Genet. 91: 1103-1107, 2012. 3. Bauche, S., O'Regan, S., Azuma, Y., Laffargue, F., McMacken, G., Sternberg, D., Brochier, G., Buon, C., Bouzidi, N., Topf, A., Lacene, E., Remerand, G., and 30 others. Impaired presynaptic high-affinity choline transporter causes a congenital myasthenic syndrome with episodic apnea. Am. J. Hum. Genet. 99: 753-761, 2016.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Andere Bezeichnung: SLC5A7

Hintergrund:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: The high-affinity choline transporter (ChT) also known as solute carrier family 5 member 7 is a protein in humans that is encoded by the SLC5A7 gene. This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 80 kDa

Gen-ID: 60482