NARF Antikörper (AA 41-353)

Produktnummer ABIN7601665

Der Kaninchen Polyklonal Anti-NARF-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, NARF in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NARF Antikörper (AA 41-353) (ABIN7601665)

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NARF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NARF Antikörper

Bindungsspezifität: AA 41-353

Verwendungszweck: Anti-NARF Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NARF Antibody Picoband® (ABIN7601665). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NARF recombinant protein (Position: H41-Q353).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barton, R. M., Worman, H. J. Prenylated prelamin A interacts with Narf, a novel nuclear protein. J. Biol. Chem. 274: 30008-30018, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NARF

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Andere Bezeichnung: NARF

Hintergrund:

Synonyms: E3 ubiquitin-protein ligase RNF169, RING finger protein 169, RING-type E3 ubiquitin transferase RNF169, RNF169, KIAA1991

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

Molekulargewicht: 52 kDa

Gen-ID: 26502