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GIT2 Antikörper (AA 400-556)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GIT2 in WB, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.
Produktnummer ABIN7601655

Kurzübersicht für GIT2 Antikörper (AA 400-556) (ABIN7601655)

Target

Alle GIT2 Antikörper anzeigen
GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Reaktivität

  • 45
  • 16
  • 14
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 36
  • 14
Kaninchen

Klonalität

  • 38
  • 12
Polyklonal

Konjugat

  • 35
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GIT2 Antikörper ist unkonjugiert

Applikation

  • 32
  • 17
  • 14
  • 10
  • 9
  • 7
  • 4
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Flow Cytometry (FACS)
  • Bindungsspezifität

    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 400-556

    Verwendungszweck

    Anti-GIT2 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-GIT2 Antibody Picoband® (ABIN7601655). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human GIT2 recombinant protein (Position: D400-S556).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.25 μg/mL, Human, Mouse
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Bagrodia, S., Bailey, D., Lenard, Z., Hart, M., Guan, J. L., Premont, R. T., Taylor, S. J., Cerione, R. A. A tyrosine-phosphorylated protein that binds to an important regulatory region on the Cool family of p21-activated kinase-binding proteins. J. Biol. Chem. 274: 22393-22400, 1999. 2. Mazaki, Y., Hashimoto, S., Okawa, K., Tsubouchi, A., Nakamura, K., Yagi, R., Yano, H., Kondo, A., Iwamatsu, A., Mizoguchi, A., Sabe, H. An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization. Molec. Biol. Cell 12: 645-662, 2001. 3. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K., Nomura, N. Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 167-174, 1995.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

    Andere Bezeichnung

    GIT2

    Hintergrund

    Synonyms: Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E, UNQ3120/PRO10272

    Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

    Background: ARF GTPase-activating protein GIT2 is an enzyme that in humans is encoded by the GIT2 gene. This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

    Molekulargewicht

    85 kDa

    Gen-ID

    9815

    UniProt

    Q14161

    Pathways

    Regulation of G-Protein Coupled Receptor Protein Signaling
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