ARHGEF12 Antikörper (AA 4-254)

Produktnummer ABIN7601615

Dieses Anti-ARHGEF12-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ARHGEF12 in ELISA, WB und FACS. Geeignet für Human und Ratte.

Kurzübersicht für ARHGEF12 Antikörper (AA 4-254) (ABIN7601615)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARHGEF12 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-ARHGEF12 Antikörper

Bindungsspezifität: AA 4-254

Verwendungszweck: Anti-LARG/ARHGEF12 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-LARG/ARHGEF12 Antibody Picoband® (ABIN7601615). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: T4-S254).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Goulimari, P., Knieling, H., Engel, U., Grosse, R. LARG and mDia1 link G-alpha-12/13 to cell polarity and microtubule dynamics. Molec. Biol. Cell 19: 30-40, 2008. 2. Kourlas, P. J., Strout, M. P., Becknell, B., Veronese, M. L., Croce, C. M., Theil, K. S., Krahe, R., Ruutu, T., Knuutila, S., Bloomfield, C. D., Caligiuri, M. A. Identification of a gene at 11q23 encoding a guanine nucleotide exchange factor: evidence for its fusion with MLL in acute myeloid leukemia. Proc. Nat. Acad. Sci. 97: 2145-2150, 2000. 3. Martz, M. K., Grabocka, E., Beeharry, N., Yen, T. J., Wedegaertner, P. B. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Molec. Biol. Cell 24: 2785-2794, 2013.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Andere Bezeichnung: ARHGEF12

Hintergrund:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 220 kDa

Gen-ID: 23365

Pathways: Neurotrophin Signalübertragung, Regulation of G-Protein Coupled Receptor Protein Signaling