Symplekin Antikörper (AA 384-1092)

Produktnummer ABIN7601558

Dieses Anti-Symplekin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Symplekin in WB, IHC, ELISA, IF, FACS und ICC. Geeignet für Human und Ratte.

Kurzübersicht für Symplekin Antikörper (AA 384-1092) (ABIN7601558)

Target: Symplekin (SYMPK)

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Symplekin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-Symplekin Antikörper

Bindungsspezifität: AA 384-1092

Verwendungszweck: Anti-Symplekin/SYMPK Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Symplekin/SYMPK Antibody Picoband® (ABIN7601558). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Symplekin/SYMPK recombinant protein (Position: K384-N1092).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alwazzan, M., Hamshere, M. G., Lennon, G. G., Brook, J. D. Six transcripts map within 200 kilobases of the myotonic dystrophy expanded repeat. Mammalian Genome 9: 485-487, 1998. 2. Keon, B. H., Schafer, S., Kuhn, C., Grund, C., Franke, W. W. Symplekin, a novel type of tight junction plaque protein. J. Cell Biol. 134: 1003-1018, 1996. 3. Kolev, N. G., Steitz, J. A. Symplekin and multiple other polyadenylation factors participate in 3-prime-end maturation of histone mRNAs. Genes Dev. 19: 2583-2592, 2005.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Symplekin

Target: Symplekin (SYMPK)

Andere Bezeichnung: SYMPK

Hintergrund:

Synonyms: N-alpha-acetyltransferase 15, NatA auxiliary subunit,Gastric cancer antigen Ga19,N-terminal acetyltransferase,NMDA receptor-regulated protein 1,Protein tubedown-1,Tbdn100,NAA15,GA19, NARG1, NATH, TBDN100,

Tissue Specificity: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. .

Background: Symplekin is a protein that in humans is encoded by the SYMPK gene. This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Molekulargewicht: 150 kDa

Gen-ID: 8189

UniProt: Q92797