SYNE2 Antikörper (AA 3765-5590)

Produktnummer ABIN7601532

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SYNE2 in ELISA, WB, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human und Affe.

Kurzübersicht für SYNE2 Antikörper (AA 3765-5590) (ABIN7601532)

Target: SYNE2 (Spectrin Repeat Containing, Nuclear Envelope 2 (SYNE2))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYNE2 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-SYNE2 Antikörper

Bindungsspezifität: AA 3765-5590

Verwendungszweck: Anti-Nesprin 2/SYNE2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Nesprin 2/SYNE2 Antibody Picoband® (ABIN7601532). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Nesprin 2/SYNE2 recombinant protein (Position: Q3765-Q5590).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Apel, E. D., Lewis, R. M., Grady, R. M., Sanes, J. R. Syne-1, a dystrophin- and Klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction. J. Biol. Chem. 275: 31986-31995, 2000. 2. Libotte, T., Zaim, H., Abraham, S., Padmakumar, V. C., Schneider, M., Lu, W., Munck, M., Hutchison, C., Wehnert, M., Fahrenkrog, B., Sauder, U., Aebi, U., Noegel, A. A., Karakesisoglou, I. Lamin A/C-dependent localization of nesprin-2, a giant scaffolder at the nuclear envelope. Molec. Biol. Cell 16: 3411-3424, 2005. 3. Maddox, D. M., Collin, G. B., Ikeda, A., Pratt, C. H., Ikeda, S., Johnson, B. A., Hurd, R. E., Shopland, L. S., Naggert, J. K., Chang, B., Krebs, M. P., Nishina, P. M. A mutation in Syne2 causes early retinal defects in photoreceptors, secondary neurons, and Mueller glia. Invest. Ophthal. Vis. Sci. 56: 3776-3787, 2015.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SYNE2

Target: SYNE2 (Spectrin Repeat Containing, Nuclear Envelope 2 (SYNE2))

Andere Bezeichnung: SYNE2

Hintergrund:

Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3

Tissue Specificity: Widely expressed.

Background: Nesprin-2 is a protein that in humans is encoded by the SYNE2 gene. The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 50-60 kDa

Gen-ID: 23224

Pathways: Maintenance of Protein Location