RLIM Antikörper (AA 369-621)

Produktnummer ABIN7601490

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RLIM in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für RLIM Antikörper (AA 369-621) (ABIN7601490)

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RLIM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-RLIM Antikörper

Bindungsspezifität: AA 369-621

Verwendungszweck: Anti-RLIM Antibody Picoband®

Produktmerkmale: Anti-RLIM Antibody Picoband® (ABIN7601490). Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RLIM recombinant protein (Position: R369-E621). Human RLIM shares 95.4% amino acid (aa) sequence identity with mouse RLIM.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bach, I., Rodriguez-Esteban, C., Carriere, C., Bhushan, A., Krones, A., Rose, D. W., Glass, C. K., Andersen, B., Belmonte, J. C. I., Rosenfeld, M. G. RLIM inhibits activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex. Nature Genet. 22: 394-399, 1999. 2. Frints, S. G. M., Ozanturk, A., Rodriguez Criado, G., Grasshoff, U., de Hoon, B., Field, M., Manouvrier-Hanu, S., Hickey, S. E., Kammoun, M., Gripp, K. W., Bauer, C., Schroeder, C., and 33 others. Pathogenic variants in E3 ubiquitin ligase RLIM/RNF12 lead to a syndromic X-linked intellectual disability and behavior disorder. Molec. Psychiat. 24: 1748-1768, 2019. 3. Gontan, C., Achame, E. M., Demmers, J., Barakat, T. S., Rentmeester, E., van IJcken, W., Grootegoed, J. A., Gribnau, J. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature 485: 386-390, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Andere Bezeichnung: RLIM

Hintergrund: E3 ubiquitin-protein ligase RLIM is an enzyme that in humans is encoded by the RLIM gene. The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molekulargewicht: 69 kDa

Gen-ID: 51132