MAG Antikörper (AA 34-605)

Produktnummer ABIN7601398

Der Kaninchen Polyklonal Anti-MAG-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, MAG in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MAG Antikörper (AA 34-605) (ABIN7601398)

Target: MAG (Myelin Associated Glycoprotein (MAG))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-MAG Antikörper

Bindungsspezifität: AA 34-605

Verwendungszweck: Anti-MAG Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MAG Antibody Picoband® (ABIN7601398). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MAG recombinant protein (Position: E34-R605).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Atwal, J. K., Pinkston-Gosse, J., Syken, J., Stawicki, S., Wu, J., Shatz, C., Tessier-Lavigne, M. PirB is a functional receptor for myelin inhibitors of axonal regeneration. Science 322: 967-970, 2008. 2. Barton, D. E., Arquint, M., Roder, J., Dunn, R., Francke, U. The myelin-associated glycoprotein gene: mapping to human chromosome 19 and mouse chromosome 7 and expression in quivering mice. Genomics 1: 107-112, 1987. 3. Liu, B. P., Fournier, A., GrandPre, T., Strittmatter, S. M. Myelin-associated glycoprotein as a functional ligand for the Nogo-66 receptor. Science 297: 1190-1193, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Andere Bezeichnung: MAG

Hintergrund:

Synonyms: Myelin-associated glycoprotein, Siglec-4a, MAG, GMA

Tissue Specificity: Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures. Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths. Detected in compact myelin (at protein level). Both isoform 1 and isoform 2 are detected in the central and peripheral nervous system

Background: MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.

Molekulargewicht: 100 kDa

Gen-ID: 4099

UniProt: P20916

Pathways: Neurotrophin Signalübertragung