ENAH Antikörper (AA 32-551)

Produktnummer ABIN7601316

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ENAH in WB, ELISA, IHC, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für ENAH Antikörper (AA 32-551) (ABIN7601316)

Target: ENAH (Enabled Homolog (ENAH))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENAH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-ENAH Antikörper

Bindungsspezifität: AA 32-551

Verwendungszweck: Anti-ENAH/MENA Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ENAH/MENA Antibody Picoband® (ABIN7601316). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ENAH/MENA recombinant protein (Position: F32-E551).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ermekova, K. S., Zambrano, N., Linn, H., Minopoli, G., Gertler, F., Russo, T., Sudol, M. The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila Enabled. J. Biol. Chem. 272: 32869-32877, 1997. 2. Laurent, V., Loisel, T. P., Harbeck, B., Wehman, A., Grobe, L., Jockusch, B. M., Wehland, J., Gertler, F. B., Carlier, M.-F. Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. J. Cell Biol. 144: 1245-1258, 1999. 3. Philippar, U., Roussos, E. T., Oser, M., Yamaguchi, H., Kim, H.-D., Giampieri, S., Wang, Y., Goswami, S., Wyckoff, J. B., Lauffenburger, D. A., Sahai, E., Condeelis, J. S., Gertler, F. B. A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis. Dev. Cell 15: 813-828, 2008.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ENAH

Target: ENAH (Enabled Homolog (ENAH))

Andere Bezeichnung: ENAH

Hintergrund:

Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097

Tissue Specificity: Expressed in fetal brain. Highly expressed in brain and placenta. Lower levels in heart, liver, thymus, kidney and lung. Located to endothelial cells and neuronal cells of the suprachiasmatic nucleus (SCN). Also detected in endothelial cells of the heart, lung and kidney. In the brain, specifically expressed in the thalamus, hippocampus and amygdala.

Background: Protein enabled homolog is a protein that in humans is encoded by the ENAH gene. This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3.

Molekulargewicht: 88 kDa

Gen-ID: 55740

UniProt: Q8N8S7