HIP1R Antikörper (AA 316-734)

Produktnummer ABIN7601290

Der Kaninchen Polyklonal Anti-HIP1R-Antikörper wurde für WB, ELISA, IHC, IF, FACS und ICC validiert. Er ist geeignet, HIP1R in Proben von Human, Ratte, Maus und Affe zu detektieren.

Kurzübersicht für HIP1R Antikörper (AA 316-734) (ABIN7601290)

Target: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIP1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-HIP1R Antikörper

Bindungsspezifität: AA 316-734

Verwendungszweck: Anti-HIP1R Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-HIP1R Antibody Picoband® (ABIN7601290). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human HIP1R recombinant protein (Position: E316-E734).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bradley, S. V., Hyun, T. S., Oravecz-Wilson, K. I., Li, L., Waldorff, E. I., Ermilov, A. N., Goldstein, S. A., Zhang, C. X., Drubin, D. G., Varela, K., Parlow, A., Dlugosz, A. A., Ross, T. S. Degenerative phenotypes caused by the combined deficiency of murine HIP1 and HIP1r are rescued by human HIP1. Hum. Molec. Genet. 16: 1279-1292, 2007. 2. Chopra, V. S., Metzler, M., Rasper, D. M., Engqvist-Goldstein, A. E. Y., Singaraja, R., Gan, L., Fichter, K. M., McCutcheon, K., Drubin, D., Nicholson, D. W., Hayden, M. R. HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin. Mammalian Genome 11: 1006-1015, 2000. 3. Engqvist-Goldstein, A. E. Y., Kessels, M. M., Chopra, V. S., Hayden, M. R., Drubin, D. G. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. J. Cell Biol. 147: 1503-1518, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu HIP1R

Target: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Andere Bezeichnung: HIP1R

Hintergrund:

Synonyms: Centriolar coiled-coil protein of 110 kDa, Centrosomal protein of 110 kDa, CP110, Cep110, CCP110, CEP110, CP110, KIAA0419

Tissue Specificity: Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

Background: Huntingtin-interacting protein 1-related protein is a protein that in humans is encoded by the HIP1R gene. Huntingtin-interacting protein 1-related protein, or HIP1R, was identified on the basis of its structural homology with Huntingtin-interacting protein 1, or HIP1. Based on its domain structure, HIP1R is a putative endocytosis-related protein. Knockdown of HIP1R impairs the endocytosis of activated epidermal growth factor receptor (EGFR) and the consequent activation of the downstream ERK and Akt proteins. Additionally, HIP1R is a component of the clathrin-coated pits and vesicles, which in part links the endocytic machinery to the actin cytoskeleton. It binds to 3-phosphoinositides via ENTH domains to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. HIP1R deficiency significantly reduces the expression of the ionotropic glutamate receptor GluA1, GluN2A, and GluN2B subunits, but not the GABAA receptor α1 subunit. Knockdown of HIP1R reduces the amplitude and frequency of the miniature excitatory postsynaptic current, but not of the miniature inhibitory postsynaptic curren. HIP1R has been identified as a protein that can target histone deacetylase-3-mediated neurodegeneration, along with other proteins like NPTX1, NFL, TEX10, and TGFFG.

Molekulargewicht: 120 kDa

Gen-ID: 9026

UniProt: O75146