MARK3 Antikörper (AA 311-648)

Produktnummer ABIN7601283

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MARK3 in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für MARK3 Antikörper (AA 311-648) (ABIN7601283)

Target: MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MARK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-MARK3 Antikörper

Bindungsspezifität: AA 311-648

Verwendungszweck: Anti-MARK3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MARK3 Antibody Picoband® (ABIN7601283). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MARK3 recombinant protein (Position: H311-D648).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ansar, M., Chung, H., Waryah, Y. M., Makrythanasis, P., Falconnet, E., Rao, A. R., Guipponi, M., Narsani, A. K., Fingerhut, R., Santoni, F. A., Ranza, E., Waryah, A. M., Bellen, H. J., Antonarakis, S. E. Visual impairment and progressive phthisis bulbi caused by recessive pathogenic variant in MARK3. Hum. Molec. Genet. 27: 2703-2711, 2018. 2. Lennerz, J. K., Hurov, J. B., White, L. S., Lewandowski, K. T., Prior, J. L., Planer, G. J., Gereau, R. W., IV, Piwnica-Worms, D., Schmidt, R. E., Piwnica-Worms, H. Loss of Par-1a/MARK3/C-TAK1 kinase leads to reduced adiposity, resistance to hepatic steatosis, and defective gluconeogenesis. Molec. Cell. Biol. 30: 5043-5056, 2010. 3. Muller, J., Ory, S., Copeland, T., Piwnica-Worms, H., Morrison, D. K. C-TAK1 regulates Ras signaling by phosphorylating the MAPK scaffold, KSR1. Molec. Cell 8: 983-993, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu MARK3

Target: MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Andere Bezeichnung: MARK3

Hintergrund:

Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097

Tissue Specificity: Expressed in fetal brain. Highly expressed in brain and placenta. Lower levels in heart, liver, thymus, kidney and lung. Located to endothelial cells and neuronal cells of the suprachiasmatic nucleus (SCN). Also detected in endothelial cells of the heart, lung and kidney. In the brain, specifically expressed in the thalamus, hippocampus and amygdala.

Background: MAP/microtubule affinity-regulating kinase 3 is an enzyme that in humans is encoded by the MARK3 gene. The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 80 kDa

Gen-ID: 4140

UniProt: P27448

Pathways: SARS-CoV-2 Protein Interaktom