NCOA2 Antikörper (AA 297-1071)

Produktnummer ABIN7601165

Dieses Anti-NCOA2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NCOA2 in WB, IHC, ELISA, IF, ICC und FACS. Geeignet für Human.

Kurzübersicht für NCOA2 Antikörper (AA 297-1071) (ABIN7601165)

Target: NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCOA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NCOA2 Antikörper

Bindungsspezifität: AA 297-1071

Verwendungszweck: Anti-NCOA2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NCOA2 Antibody Picoband® (ABIN7601165). Tested in WB, IF, IHC, ICC, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NCOA2 recombinant protein (Position: E297-E1071). Human NCOA2 shares 93.4% and 90.8% amino acid (aa) sequence identity with mouse and rat NCOA2, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, Human
1. Aikawa, Y., Katsumoto, T., Zhang, P., Shima, H., Shino, M., Terui, K., Ito, E., Ohno, H., Stanley, E. R., Singh, H., Tenen, D. G., Kitabayashi, I. PU.1-mediated upregulation of CSF1R is crucial for leukemia stem cell potential induced by MOZ-TIF2. (Letter) Nature Med. 16: 580-585, 2010. 2. Carapeti, M., Aguiar, R. C., Goldman, J. M., Cross, N. C. P. A novel fusion between MOZ and the nuclear receptor coactivator TIF2 in acute myeloid leukemia. Blood 91: 3127-3133, 1998. 3. Chen, S. L., Dowhan, D. H., Hosking, B. M., Muscat, G. E. O. The steroid receptor coactivator, GRIP-1, is necessary for MEF-2C-dependent gene expression and skeletal muscle differentiation. Genes Dev. 14: 1209-1228, 2000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NCOA2

Target: NCOA2 (Nuclear Receptor Coactivator 2 (NCOA2))

Andere Bezeichnung: NCOA2

Hintergrund:

Synonyms: NCOA2, BHLHE75, SRC2, TIF2, Nuclear receptor coactivator 2, NCoA-2, Class E basic helix-loop-helix protein 75, bHLHe75, Transcriptional intermediary factor 2, hTIF2

Background: The nuclear receptor coactivator 2 also known as NCoA-2 is a protein that in humans is encoded by the NCOA2 gene. The protein encoded by this gene functions as a transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid, and vitamin D receptors. The encoded protein acts as an intermediary factor for the ligand-dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. This gene has been found to be involved in translocations that result in fusions with other genes in various cancers, including the lysine acetyltransferase 6A (KAT6A) gene in acute myeloid leukemia, the ETS variant 6 (ETV6) gene in acute lymphoblastic leukemia, and the hes related family bHLH transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal chondrosarcoma. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 160-170 kDa

Gen-ID: 10499

UniProt: Q15596

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling