CD43 Antikörper (AA 272-400)

Produktnummer ABIN7601042

Dieses Anti-CD43-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD43 in FACS, WB und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CD43 Antikörper (AA 272-400) (ABIN7601042)

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 4I3

Produktdetails anti-CD43 Antikörper

Bindungsspezifität: AA 272-400

Verwendungszweck: Anti-CD43/SPN Antibody Picoband® (monoclonal, 4I3)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) (ABIN7601042). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequence identity with mouse and rat CD43, respectively.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fukuda M, Carlsson SR (1987). "Leukosialin, a major sialoglycoprotein on human leukocytes as differentiation antigens.". Med. Biol. 64 (6): 335-43. 2. Rogaev EI, Keryanov SA (1993). "Unusual variability of the complex dinucleotide repeat block at the SPN locus.". Hum. Mol. Genet. 1 (8): 657. 3. Rosenstein Y, Park JK, Hahn WC, et al. (1992). "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1.". Nature 354 (6350): 233-5.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: SPN

Hintergrund:

Synonyms: Leukosialin, GPL115, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, CD43 cytoplasmic tail, CD43-ct, CD43ct, SPN, CD43

Tissue Specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Background: CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Molekulargewicht: 115 kDa

Gen-ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity