PPP1R35 Antikörper (AA 27-253)

Produktnummer ABIN7601004

Produktdetails anti-PPP1R35 Antikörper

Target: PPP1R35 (Protein Phosphatase 1, Regulatory Subunit 35 (PPP1R35))

Bindungsspezifität: AA 27-253

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPP1R35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Verwendungszweck: Anti-C7orf47/PPP1R35 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-C7orf47/PPP1R35 Antibody Picoband® (ABIN7601004). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human C7orf47/PPP1R35 recombinant protein (Position: E27-A253).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fong, C. S., Ozaki, K., Tsou, M.-F. B. PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. Molec. Biol. Cell 29: 2801-2808, 2018. 2. Gross, M. B. Personal Communication. Baltimore, Md. 7/1/2020. 3. Hendrickx, A., Beullens, M., Ceulemans, H., Abt, T. D., Van Eynde, A., Nicolaescu, E., Lesage, B., Bollen, M. Docking motif-guided mapping of the interactome of protein phosphatase-1. Chem. Biol. 16: 365-371, 2009.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PPP1R35

Target: PPP1R35 (Protein Phosphatase 1, Regulatory Subunit 35 (PPP1R35))

Andere Bezeichnung: PPP1R35 (PPP1R35 Produkte)

Hintergrund:

Synonyms: Transmembrane protein 240,TMEM240,C1orf70,

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: PPP1R35 is a putative regulator of protein phosphatase-1. PPP1R35 is a centrosomal protein that regulates centriole elongation and centriole-to-centrosome conversion. Predicted to enable phosphatase binding activity and protein phosphatase inhibitor activity. Predicted to be involved in negative regulation of phosphatase activity.

Molekulargewicht: 30 kDa

Gen-ID: 221908