SHARPIN Antikörper (AA 25-325)

Produktnummer ABIN7600904

Dieser Anti-SHARPIN Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von SHARPIN in WB, ELISA, IHC und FACS. Geeignet für Human, Ratte und Maus.

Kurzübersicht für SHARPIN Antikörper (AA 25-325) (ABIN7600904)

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SHARPIN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-SHARPIN Antikörper

Bindungsspezifität: AA 25-325

Verwendungszweck: Anti-SHARPIN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SHARPIN Antibody Picoband® (ABIN7600904). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SHARPIN recombinant protein (Position: H25-R325). Human SHARPIN shares 72.3% and 72.6% amino acid (aa) sequence identity with mouse and rat SHARPIN, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Ang, R. L., Chan, M., Legarda, D., Sundberg, J. P., Sun, S. C., Gillespie, V. L., Chun, N., Heeger, P. S., Xiong, H., Lira, S. A., Ting, A. T. Immune dysregulation in SHARPIN-deficient mice is dependent on CYLD-mediated cell death. Proc. Nat. Acad. Sci. 118: e2001602118, 2021. 2. Daigo, Y., Takayama, I., Ward, S. M., Sanders, K. M., Fujino, M. A. Novel human and mouse genes encoding a shank-interacting protein and its upregulation in gastric fundus of W/W(v) mouse. J. Gastroent. Hepatol. 18: 712-718, 2003. 3. Gerlach, B., Cordier, S. M., Schmukle, A. C., Emmerich, C. H., Rieser, E., Haas, T. L., Webb, A. I., Rickard, J. A., Anderton, H., Wong, W. W.-L., Nachbur, U., Gangoda, L., Warnken, U., Purcell, A. W., Silke, J., Walczak, H. Linear ubiquitination prevents inflammation and regulates immune signalling. Nature 471: 591-596, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SHARPIN

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Andere Bezeichnung: SHARPIN

Hintergrund:

Synonyms: SHARPIN, SIPL1, PSEC0216, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1

Background: Enables polyubiquitin modification-dependent protein binding activity. Involved in defense response to bacterium, protein linear polyubiquitination, and regulation of signal transduction. Located in cytosol. Part of LUBAC complex.

Molekulargewicht: 40 kDa

Gen-ID: 81858

UniProt: Q9H0F6