MMS19 Antikörper (AA 239-998)

Produktnummer ABIN7600802

Dieses Anti-MMS19-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MMS19 in WB, ELISA, IF, IHC, ICC und FACS. Geeignet für Human und Maus.

Kurzübersicht für MMS19 Antikörper (AA 239-998) (ABIN7600802)

Target: MMS19 (MMS19 Nucleotide Excision Repair Homolog (MMS19))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MMS19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-MMS19 Antikörper

Bindungsspezifität: AA 239-998

Verwendungszweck: Anti-MMS19 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MMS19 Antibody Picoband® (ABIN7600802). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MMS19 recombinant protein (Position: E239-R998).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gari, K., Leon Ortiz, A. M., Borel, V., Flynn, H., Skehel, J. M., Boulton, S. J. MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism. Science 337: 243-245, 2012. 2. Hatfield, M. D., Reis, A. M. C., Obeso, D., Cook, J. R., Thompson, D. M., Rao, M., Friedberg, E. C., Queimado, L. Identification of MMS19 domains with distinct functions in NER and transcription. DNA Repair 5: 914-924, 2006. 3. Ito, S., Tan, L. J., Andoh, D., Narita, T., Seki, M., Hirano, Y., Narita, K., Kuraoka, I., Hiraoka, Y., Tanaka, K. MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation. Molec. Cell 39: 632-640, 2010.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu MMS19

Target: MMS19 (MMS19 Nucleotide Excision Repair Homolog (MMS19))

Andere Bezeichnung: MMS19

Hintergrund:

Synonyms: ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL2, HUB

Tissue Specificity: Brain, neural-specific.

Background: MMS19 nucleotide excision repair protein homolog is a protein that in humans is encoded by the MMS19 gene. MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

Molekulargewicht: 113 kDa

Gen-ID: 64210

UniProt: Q96T76

Pathways: Nuclear Hormone Receptor Binding