NAB2 Antikörper (AA 235-525)

Produktnummer ABIN7600786

Der Kaninchen Polyklonal Anti-NAB2-Antikörper wurde für WB, ELISA, ICC, IF und FACS validiert. Er ist geeignet, NAB2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NAB2 Antikörper (AA 235-525) (ABIN7600786)

Target: NAB2 (NGFI-A Binding Protein 2 (EGR1 Binding Protein 2) (NAB2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-NAB2 Antikörper

Bindungsspezifität: AA 235-525

Verwendungszweck: Anti-NAB2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NAB2 Antibody Picoband® (ABIN7600786). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NAB2 recombinant protein (Position: D235-Q525). Human NAB2 shares 96.6% amino acid (aa) sequence identity with mouse NAB2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Chmielecki, J., Crago, A. M., Rosenberg, M., O'Connor, R., Walker, S. R., Ambrogio, L., Auclair, D., McKenna, A., Heinrich, M. C., Frank, D. A., Meyerson, M. Whole-exome sequencing identifies a recurrent NAB2-STAT6 fusion in solitary fibrous tumors. Nature Genet. 45: 131-135, 2013. 2. Le, N., Nagarajan, R., Wang, J. Y. T., Svaren, J., LaPash, C., Araki, T., Schmidt, R. E., Milbrandt, J. Nab proteins are essential for peripheral nervous system myelination. Nature Neurosci. 8: 932-940, 2005. 3. Robinson, D. R., Wu, Y.-M., Kalyana-Sundaram, S., Cao, X., Lonigro, R. J., Sung, Y.-S., Chen, C.-L., Zhang, L., Wang, R., Su, F., Iyer, M. K., Roychowdhury, S., and 9 others. Identification of recurrent NAB2-STAT6 gene fusions in solitary fibrous tumor by integrative sequencing. Nature Genet. 45: 180-185, 2013.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NAB2

Target: NAB2 (NGFI-A Binding Protein 2 (EGR1 Binding Protein 2) (NAB2))

Andere Bezeichnung: NAB2

Hintergrund:

Synonyms: NAB2, MADER, NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER

Background: NGFI-A-binding protein 2 also known as EGR-1-binding protein 2 or melanoma-associated delayed early response protein (MADER) is a protein that in humans is encoded by the NAB2 gene. This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Molekulargewicht: 57 kDa

Gen-ID: 4665

UniProt: Q15742