NOTCH2 Antikörper (AA 2194-2471)

Produktnummer ABIN7600664

Dieses Anti-NOTCH2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NOTCH2 in WB, ELISA, IHC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für NOTCH2 Antikörper (AA 2194-2471) (ABIN7600664)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTCH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-NOTCH2 Antikörper

Bindungsspezifität: AA 2194-2471

Verwendungszweck: Anti-NOTCH2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NOTCH2 Antibody Picoband® (ABIN7600664). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NOTCH2 recombinant protein (Position: H2194-A2471).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL
Immunohistochemistry, 2-5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Blaumueller, C. M., Qi, H., Zagouras, P., Artavanis-Tsakonas, S. Fixed cleavage of Notch leads to a heterodimeric receptor on the plasma membrane. Cell 90: 281-291, 1997. 2. Gao, X., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Gridley, T. Assignment of the murine Notch2 and Notch3 genes to chromosomes 3 and 17. Genomics 49: 160-161, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: NOTCH2

Hintergrund:

Synonyms: Neurogenic locus notch homolog protein 2, Notch 2, Hn2, Notch 2 extracellular truncation, N2ECD, Notch 2 intracellular domain, N2ICD, NOTCH2

Tissue Specificity: Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.

Background: Neurogenic locus notch homolog protein 2 also known as notch 2 is a protein that in humans is encoded by the NOTCH2 gene. It is mapped to 1p12. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 110 kDa, 300 kDa

Gen-ID: 4853

UniProt: Q04721

Pathways: Notch Signalweg, Stem Cell Maintenance