Keratin 2 Antikörper (AA 213-332)

Produktnummer ABIN7600644

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Keratin 2 in WB, IHC, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Keratin 2 Antikörper (AA 213-332) (ABIN7600644)

Target: Keratin 2 (KRT2)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Keratin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Keratin 2 Antikörper

Bindungsspezifität: AA 213-332

Verwendungszweck: Anti-Cytokeratin 2e/KRT2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cytokeratin 2e/KRT2 Antibody Picoband® (ABIN7600644). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cytokeratin 2e/KRT2 recombinant protein (Position: L213-N332).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Basarab, T., Smith, F. J. D., Jolliffe, V. M. L., McLean, W. H. I., Neill, S., Rustin, M. H. A., Eady, R. A. J. Ichthyosis bullosa of Siemens: report of a family with evidence of a keratin 2e mutation, and a review of the literature. Brit. J. Derm. 140: 689-695, 1999. 2. Collin, C., Moll, R., Kubicka, S., Ouhayoun, J.-P., Franke, W. W. Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation. Exp. Cell Res. 202: 132-141, 1992. 3. Kremer, H., Zeeuwen, P., McLean, W. H. I., Mariman, E. C. M., Lane, E. B., van de Kerkhof, P. C. M., Ropers, H.-H., Steijlen, P. M. Ichthyosis bullosa of Siemens is caused by mutations in the keratin 2e gene. J. Invest. Derm. 103: 286-289, 1994.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Keratin 2

Target: Keratin 2 (KRT2)

Andere Bezeichnung: KRT2

Hintergrund:

Synonyms: N-alpha-acetyltransferase 15, NatA auxiliary subunit,Gastric cancer antigen Ga19,N-terminal acetyltransferase,NMDA receptor-regulated protein 1,Protein tubedown-1,Tbdn100,NAA15,GA19, NARG1, NATH, TBDN100,

Tissue Specificity: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. .

Background: Keratin 2A also known as keratin 2E or keratin 2 is a protein that in humans is encoded by the KRT2A gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Molekulargewicht: 65 kDa

Gen-ID: 3849

UniProt: P35908