MAD2L1BP Antikörper (AA 20-274)

Produktnummer ABIN7600535

Dieses Anti-MAD2L1BP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MAD2L1BP in WB, ELISA und FACS. Geeignet für Human.

Kurzübersicht für MAD2L1BP Antikörper (AA 20-274) (ABIN7600535)

Target: MAD2L1BP (MAD2L1 Binding Protein (MAD2L1BP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAD2L1BP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-MAD2L1BP Antikörper

Bindungsspezifität: AA 20-274

Verwendungszweck: Anti-MAD2L1BP Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MAD2L1BP Antibody Picoband® (ABIN7600535). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MAD2L1BP recombinant protein (Position: Y20-E274).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alfieri, C., Chang, L., Barford, D. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559: 274-278, 2018. 2. Date, D. A., Burrows, A. C., Venere, M., Jackson, M. W., Summers, M. K. Coordinated regulation of p31(Comet) and Mad2 expression is required for cellular proliferation. Cell Cycle 12: 3824-3832, 2013. 3. Eytan, E., Wang, K., Miniowitz-Shemtov, S., Sitry-Shevah, D., Kaisari, S., Yen, T. J., Liu, S.-T., Hershko, A. Disassembly of mitotic checkpoint complexes by the joint action of the AAA-ATPase TRIP13 and p13(comet). Proc. Nat. Acad. Sci. 111: 12019-12024, 2014.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu MAD2L1BP

Target: MAD2L1BP (MAD2L1 Binding Protein (MAD2L1BP))

Andere Bezeichnung: MAD2L1BP

Hintergrund:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: MAD2L1-binding protein is a protein that in humans is encoded by the MAD2L1BP gene. The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molekulargewicht: 37 kDa

Gen-ID: 9587

UniProt: Q15013