Histone H1 Antikörper (AA 20-159)

Produktnummer ABIN7600521

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Histone H1 in WB, ELISA, IHC, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Histone H1 Antikörper (AA 20-159) (ABIN7600521)

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-Histone H1 Antikörper

Bindungsspezifität: AA 20-159

Verwendungszweck: Anti-Histone H1.0/H1F0 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Histone H1.0/H1F0 Antibody Picoband® (ABIN7600521). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Doenecke D, Tönjes R (February 1986). "Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene". Journal of Molecular Biology. 187 (3): 461-4. 2. Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik KH, Doenecke D (June 1993). "All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6". Genomics. 16 (3): 649-54.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Histone H1

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Andere Bezeichnung: H1F0

Hintergrund:

Synonyms: Histone H1.0, Histone H1', Histone H1 (0), H1F0, H1FV

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: H1 histone family, member 0?is a member of the?histone?family of nuclear?proteins?which are a component of?chromatin. In humans, this protein is encoded by the?H1F0?gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Molekulargewicht: 24 kDa

Gen-ID: 3005

UniProt: P07305