ALT Antikörper (AA 2-496)

Produktnummer ABIN7600506

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ALT in WB, IHC, ELISA, ICC, IF und FACS. Er zeigt eine Reaktivität gegenüber Ratte und Maus.

Kurzübersicht für ALT Antikörper (AA 2-496) (ABIN7600506)

Target: ALT (Alanine Aminotransferase (ALT))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-ALT Antikörper

Bindungsspezifität: AA 2-496

Verwendungszweck: Anti-Alanine Transaminase/Gpt Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Alanine Transaminase/Gpt Antibody Picoband® (ABIN7600506). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Alanine Transaminase/Gpt recombinant protein (Position: A2-S496).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Astrin, K. H., Arredondo-Vega, F. X., Desnick, R. J., Smith, M. Assignment of the gene for cytosolic alanine aminotransferase (AAT1) to human chromosome 8. Ann. Hum. Genet. 46: 125-133, 1982. 2. Chen, S.-H., Giblett, E. R. Polymorphism of soluble glutamic-pyruvic transaminase: a new genetic marker in man. Science 173: 148-149, 1971. 3. Cook, P. J. L., Jeremiah, S. J., Buckton, K. E. Exclusion mapping of GPT. (Abstract) Cytogenet. Cell Genet. 32: 258 only, 1982.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ALT

Target: ALT (Alanine Aminotransferase (ALT))

Andere Bezeichnung: Gpt

Hintergrund:

Synonyms: Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, PITX2, ARP1, RGS, RIEG, RIEG1

Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.

Background: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1), also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase

Molekulargewicht: 55 kDa

Gen-ID: 76282