TRIM63 Antikörper (AA 2-329)

Produktnummer ABIN7600489

Der Kaninchen Polyklonal Anti-TRIM63-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, TRIM63 in Proben von Human und Affe zu detektieren.

Kurzübersicht für TRIM63 Antikörper (AA 2-329) (ABIN7600489)

Target: TRIM63 (Tripartite Motif Containing 63 (TRIM63))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM63 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TRIM63 Antikörper

Bindungsspezifität: AA 2-329

Verwendungszweck: Anti-MURF1/TRIM63 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MURF1/TRIM63 Antibody Picoband® (ABIN7600489). Tested in ELISA, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MURF1/TRIM63 recombinant protein (Position: D2-E329).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Monkey
ELISA, 0.1-0.5 μg/mL, -
1. Bodine, S. C., Latres, E., Baumhueter, S., Lai, V. K.-M., Nunez, L., Clarke, B. A., Poueymirou, W. T., Panaro, F. J., E., Dharmarajan, K., Pan, Z.-Q., Valenzuela, D. M., DeChiara, T. M., Stitt, T. N., Yancopoulos, G. D., Glass, D. J. Identification of ubiquitin ligases required for skeletal muscle atrophy. Science 294: 1704-1708, 2001. 2. Cai, D., Frantz, J. D., Tawa, N. E., Jr., Melendez, P. A., Oh, B.-C., Lidov, H. G. W., Hasselgren, P.-O., Frontera, W. R., Lee, J., Glass, D. J., Shoelson, S. E. IKK-beta/NF-kappa-B activation causes severe muscle wasting in mice. Cell 119: 285-298, 2004. 3. Centner, T., Yano, J., Kimura, E., McElhinny, A. S., Pelin, K., Witt, C. C., Bang, M.-L., Trombitas, K., Granzier, H., Gregorio, C. C., Sorimachi, H., Labeit, S. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J. Molec. Biol. 306: 717-726, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu TRIM63

Target: TRIM63 (Tripartite Motif Containing 63 (TRIM63))

Andere Bezeichnung: TRIM63

Hintergrund:

Synonyms: Tripartite motif-containing protein 6, RING finger protein 89, RING-type E3 ubiquitin transferase TRIM6, TRIM6, RNF89

Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Background: E3 ubiquitin-protein ligase TRIM63 is an enzyme that in humans is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.

Molekulargewicht: 40 kDa

Gen-ID: 84676

UniProt: Q969Q1