NDRG1 Antikörper (AA 2-220)

Produktnummer ABIN7600474

Der Kaninchen Polyklonal Anti-NDRG1-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, NDRG1 in Proben von Human zu detektieren.

Kurzübersicht für NDRG1 Antikörper (AA 2-220) (ABIN7600474)

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NDRG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NDRG1 Antikörper

Bindungsspezifität: AA 2-220

Verwendungszweck: Anti-NDRG1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NDRG1 Antibody Picoband® (ABIN7600474). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NDRG1 recombinant protein (Position: S2-N220).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hunter, M., Bernard, R., Freitas, E., Boyer, A., Morar, B., Martins, I. J., Tournev, I., Jordanova, A., Guergelcheva, V., Ishpekova, B., Kremensky, I., Nicholson, G., Schlotter, B., Lochmuller, H., Voit, T., Colomer, J., Thomas, P. K., Levy, N., Kalaydjieva, L. Mutation screening of the N-myc downstream-regulated gene 1 (NDRG1) in patients with Charcot-Marie-Tooth disease. Hum. Mutat. 22: 129-135, 2003. 2. Okamoto, Y., Goksungur, M. T., Pehlivan, D., Beck, C. R., Gonzaga-Jauregui, C., Muzny, D. M., Atik, M. M., Carvalho, C. M. B., Matur, Z., Bayraktar, S., Boone, P. M., Akyuz, K., Gibbs, R. A., Battaloglu, E., Parman, Y., Lupski, J. R. Exonic duplication CNV of NDRG1 associated with autosomal-recessive HMSN-Lom/CMT4D. Genet. Med. 16: 386-394, 2014. Note: Erratum: Genet. Med. 16: 203 only, 2014. 3. Okuda, T., Higashi, Y., Kokame, K., Tanaka, C., Kondoh, H., Miyata, T. Ndrg1-deficient mice exhibit a progressive demyelinating disorder of peripheral nerves. Molec. Cell. Biol. 24: 3949-3956, 2004.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NDRG1

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Andere Bezeichnung: NDRG1

Hintergrund:

Synonyms: Protein NDRG1, Differentiation-related gene 1 protein, DRG-1, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, RTP, Rit42, NDRG1, CAP43, DRG1, RTP

Tissue Specificity: Ubiquitous, expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

Background: Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation.

Molekulargewicht: 46 kDa

Gen-ID: 10397

UniProt: Q92597