NUB1 Antikörper (AA 190-543)

Produktnummer ABIN7600427

Dieses Anti-NUB1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NUB1 in WB, IHC, ELISA, IF, FACS und ICC. Geeignet für Human, Maus, Affe und Ratte.

Kurzübersicht für NUB1 Antikörper (AA 190-543) (ABIN7600427)

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Reaktivität: Human, Maus, Affe, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-NUB1 Antikörper

Bindungsspezifität: AA 190-543

Verwendungszweck: Anti-NUB1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NUB1 Antibody Picoband® (ABIN7600427). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NUB1 recombinant protein (Position: K190-D543).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Akey, D. T., Zhu, X., Dyer, M., Li, A., Sorensen, A., Blackshaw,S., Fukuda-Kamitani, T., Daiger, S. P., Craft, C. M., Kamitani, T., Sohocki, M. M. The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1. Hum. Molec. Genet. 11: 2723-2733, 2002. Note: Erratum: Hum. Molec. Genet. 12: 451 only, 2003. 2. Kito, K., Yeh, E. T. H., Kamitani, T. NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression. J. Biol. Chem. 276: 20603-20609, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NUB1

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Andere Bezeichnung: NUB1

Hintergrund:

Synonyms: RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, NOVA1

Tissue Specificity: Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney.

Background: NEDD8 µLtimate buster 1 is a protein that in humans is encoded by the NUB1 gene. This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.

Molekulargewicht: 75 kDa

Gen-ID: 51667

UniProt: Q9Y5A7