NOS1 Antikörper (AA 19-1320)

Produktnummer ABIN7600399

Der Kaninchen Polyklonal Anti-NOS1-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, NOS1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für NOS1 Antikörper (AA 19-1320) (ABIN7600399)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NOS1 Antikörper

Bindungsspezifität: AA 19-1320

Verwendungszweck: Anti-nNOS (neuronal)/NOS1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-nNOS (neuronal)/NOS1 Antibody Picoband® (ABIN7600399). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human nNOS (neuronal)/NOS1 recombinant protein (Position: R19-E1320).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: NOS1 Nitric oxide synthase 1 (neuronal)". 2. Shteyer, E., Edvardson, S., Wynia-Smith, S. L., Pierri, C. L., Zangen, T., Hashavya, S., Begin, M., Yaacov, B., Cinamon, Y., Koplewitz, B. Z., Vromen, A., Elpeleg, O., Smith, B. C. Truncating mutation in the nitric oxide synthase 1 gene is associated with infantile achalasia. Gastroenterology 148: 533-536, 2015. 3. Xu, W., Gorman, P., Sheer, D., Bates, G., Kishimoto, J., Lizhi, L., Emson, P. Regional localization of the gene coding for human brain nitric oxide synthase (NOS1) to 12q24.2-24.31 by fluorescent in situ hybridization. Cytogenet. Cell Genet. 64: 62-63, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Andere Bezeichnung: NOS1

Hintergrund:

Synonyms: Nitric oxide synthase, brain,1.14.13.39,Constitutive NOS,NC-NOS,N

Tissue Specificity: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Background: Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

Molekulargewicht: 161 kDa

Gen-ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction