Prosaposin Antikörper (AA 182-479)

Produktnummer ABIN7600369

Der Kaninchen Polyklonal Anti-Prosaposin-Antikörper wurde für WB, IHC, ELISA, IF, ICC und FACS validiert. Er ist geeignet, Prosaposin in Proben von Human zu detektieren.

Kurzübersicht für Prosaposin Antikörper (AA 182-479) (ABIN7600369)

Target: Prosaposin (PSAP)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prosaposin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Prosaposin Antikörper

Bindungsspezifität: AA 182-479

Verwendungszweck: Anti-PSAP Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PSAP Antibody Picoband® (ABIN7600369). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PSAP recombinant protein (Position: Q182-I479).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Azuma, N., O'Brien, J. S., Moser, H. W., Kishimoto, Y. Stimulation of acid ceramidase activity by saposin D. Arch. Biochem. Biophys. 311: 354-357, 1994. 2. Bar-Am, I., Avivi, L., Horowitz, M. Assignment of the human prosaposin gene (PSAP) to 10q22.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 72: 316-318, 1996. 3. Bradova, V., Smid, F., Ulrich-Bott, B., Roggendorf, W., Paton, B. C., Harzer, K. Prosaposin deficiency: further characterization of the sphingolipid activator protein-deficient sibs. Multiple glycolipid elevations (including lactosylceramidosis), partial enzyme deficiencies and ultrastructure of the skin in this generalized sphingolipid storage disease. Hum. Genet. 92: 143-152, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Prosaposin

Target: Prosaposin (PSAP)

Andere Bezeichnung: PSAP

Hintergrund:

Synonyms: Tafazzin, Protein G4.5, TAZ, EFE2, G4.5

Tissue Specificity: High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.

Background: This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molekulargewicht: 70 kDa

Gen-ID: 5660

UniProt: P07602

Pathways: Positive Regulation of Endopeptidase Activity