DDIT3 Antikörper (AA 17-168)

Produktnummer ABIN7600249

Der Kaninchen Polyklonal Anti-DDIT3-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, DDIT3 in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für DDIT3 Antikörper (AA 17-168) (ABIN7600249)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDIT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-DDIT3 Antikörper

Bindungsspezifität: AA 17-168

Verwendungszweck: Anti-Ddit3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Ddit3 Antibody Picoband® (ABIN7600249). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Ddit3 recombinant protein (Position: E17-A168).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Aman, P., Ron, D., Mandahl, N., Fioretos, T., Heim, S., Arheden, K., Willen, H., Rydholm, A., Mitelman, F. Rearrangement of the transcription factor gene CHOP in myxoid liposarcomas with t(12,16)(q13,p11). Genes Chromosomes Cancer 5: 278-285, 1992. 2. Crozat, A., Aman, P., Mandahl, N., Ron, D. Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma. Nature 363: 640-644, 1993. 3. Endo, M., Mori, M., Akira, S., Gotoh, T. C/EBP homologous protein (CHOP) is crucial for the induction of caspase-11 and the pathogenesis of lipopolysaccharide-induced inflammation. J. Immun. 176: 6245-6253, 2006.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Andere Bezeichnung: Ddit3

Hintergrund:

Synonyms: DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, DDIT3, CHOP, CHOP10, GADD153

Tissue Specificity: Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.

Background: DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotictranscription factor that is encoded by the DDIT3 gene. It is mapped to 12q13.3. This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Molekulargewicht: 29 kDa

Gen-ID: 13198

UniProt: P35639

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis