NUP54 Antikörper (AA 166-493)

Produktnummer ABIN7600231

Der Kaninchen Polyklonal Anti-NUP54-Antikörper wurde für WB, ELISA, FACS, ICC, IF und IHC validiert. Er ist geeignet, NUP54 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für NUP54 Antikörper (AA 166-493) (ABIN7600231)

Target: NUP54 (Nucleoporin 54kDa (NUP54))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUP54 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-NUP54 Antikörper

Bindungsspezifität: AA 166-493

Verwendungszweck: Anti-NUP54 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NUP54 Antibody Picoband® (ABIN7600231). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NUP54 recombinant protein (Position: Q166-H493).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chug, H., Trakhanov, S., Hulsmann, B. B., Pleiner, T., Gorlich, D. Crystal structure of the metazoan Nup62-Nup58-Nup54 nucleoporin complex. Science 350: 106-110, 2015. 2. Hu, T., Guan, T., Gerace, L. Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins. J. Cell Biol. 134: 589-601, 1996.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NUP54

Target: NUP54 (Nucleoporin 54kDa (NUP54))

Andere Bezeichnung: NUP54

Hintergrund:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: Nucleoporin 54 (Nup54) is a protein that in humans is encoded by the NUP54 gene. The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene.

Molekulargewicht: 58 kDa

Gen-ID: 53371

UniProt: Q7Z3B4

Pathways: SARS-CoV-2 Protein Interaktom