SEC61B Antikörper (AA 16-96)

Produktnummer ABIN7600188

Der Kaninchen Polyklonal Anti-SEC61B-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, SEC61B in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für SEC61B Antikörper (AA 16-96) (ABIN7600188)

Target: SEC61B (Sec61 beta Subunit (SEC61B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SEC61B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-SEC61B Antikörper

Bindungsspezifität: AA 16-96

Verwendungszweck: Anti-SEC61B Antibody Picoband®

Produktmerkmale: Anti-SEC61B Antibody Picoband® (ABIN7600188). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SEC61B recombinant protein (Position: R16-S96). Human SEC61B shares 98.8% amino acid (aa) sequence identity with mouse SEC61B.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bebok, Z., Mazzochi, C., King, S. A., Hong, J. S., Sorscher, E. J. The mechanism underlying cystic fibrosis transmembrane conductance regulator transport from the endoplasmic reticulum to the proteasome includes Sec61-beta and a cytosolic, deglycosylated intermediary. J. Biol. Chem. 273: 29873-29878, 1998. 2. Besse, W., Dong, K., Choi, J., Punia, S., Fedeles, S. V., Choi, M., Gallagher, A.-R., Huang, E. B., Gulati, A., Knight, J., Mane, S., Tahvanainen, E., Tahvanainen, P., Sanna-Cherchi, S., Lifton, R. P., Watnick, T., Pei, Y. P., Torres, V. E., Somlo, S. Isolated polycystic liver disease genes define effectors of polycystin-1 function. J. Clin. Invest. 127: 1772-1785, 2017. Note: Erratum: J. Clin. Invest. 127: 3558 only, 2017. 3. Chen, Y., Le Caherec, F., Chuck, S. L. Calnexin and other factors that alter translocation affect the rapid binding of ubiquitin to apoB in the Sec61 complex. J. Biol. Chem. 273: 11887-11894, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SEC61B

Target: SEC61B (Sec61 beta Subunit (SEC61B))

Andere Bezeichnung: SEC61B

Hintergrund: Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.

Molekulargewicht: 15 kDa

Gen-ID: 10952

UniProt: P60468

Pathways: Protein targeting to Nucleus