TRIT1 Antikörper (AA 16-415)

Produktnummer ABIN7600178

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TRIT1 in WB, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TRIT1 Antikörper (AA 16-415) (ABIN7600178)

Target: TRIT1 (tRNA Isopentenyltransferase 1 (TRIT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-TRIT1 Antikörper

Bindungsspezifität: AA 16-415

Verwendungszweck: Anti-IPT/TRIT1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-IPT/TRIT1 Antibody Picoband® (ABIN7600178). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human IPT/TRIT1 recombinant protein (Position: L16-K415).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Golovko, A., Hjalm, G., Sitbon, F., Nicander, B. Cloning of a human tRNA isopentenyl transferase. Gene 258: 85-93, 2000. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/19/2018. 3. Kernohan, K. D., Dyment, D. A., Pupavac, M., Cramer, Z., McBride, A., Bernard, G., Straub, I., Tetreault, M., Hartley, T., Huang, L., Sell, E., Majewski, J., and 14 others. Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene. Hum. Mutat. 38: 511-516, 2017.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu TRIT1

Target: TRIT1 (tRNA Isopentenyltransferase 1 (TRIT1))

Andere Bezeichnung: TRIT1

Hintergrund:

Synonyms: Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8,

Tissue Specificity: Ubiquitous.

Background: tRNA isopentenyltransferase, mitochondrial is an enzyme that in humans is encoded by the TRIT1 gene. This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional.

Molekulargewicht: 53 kDa

Gen-ID: 54802

UniProt: Q9H3H1