HNRNPL Antikörper (AA 152-569)

Produktnummer ABIN7600130

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HNRNPL in WB, IHC, ELISA, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für HNRNPL Antikörper (AA 152-569) (ABIN7600130)

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HNRNPL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-HNRNPL Antikörper

Bindungsspezifität: AA 152-569

Verwendungszweck: Anti-hnRNP L/HNRNPL Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-hnRNP L/HNRNPL Antibody Picoband® (ABIN7600130). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human hnRNP L/HNRNPL recombinant protein (Position: N152-N569).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hamilton, B. J., Nichols, R. C., Tsukamoto, H., Boado, R. J., Pardridge, W. M., Rigby, W. F. C. hnRNP A2 and hnRNP L bind the 3-prime UTR of glucose transporter 1 mRNA and exist as a complex in vivo. Biochem. Biophys. Res. Commun. 261: 646-651, 1999. 2. Li, Z., Chao, T.-C., Chang, K.-Y., Lin, N., Patil, V. S., Shimizu, C., Head, S. R., Burns, J. C., Rana, T. M. The long noncoding RNA THRIL regulates TNF-alpha expression through its interaction with hnRNPL. Proc. Nat. Acad. Sci. 111: 1002-1007, 2014. 3. Pinol-Roma, S., Swanson, M. S., Gall, J. G., Dreyfuss, G. A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts. J. Cell Biol. 109: 2575-2587, 1989.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu HNRNPL

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Andere Bezeichnung: HNRNPL

Hintergrund:

Synonyms: Heterogeneous nuclear ribonucleoprotein L,hnRNP L,HNRNPL,HNRPL,P/OKcl.14,

Tissue Specificity: Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.

Background: Heterogeneous nuclear ribonucleoprotein L is a protein that in humans is encoded by the HNRNPL gene. Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 68 kDa

Gen-ID: 3191

UniProt: P14866