NSMCE2 Antikörper (AA 14-228)

Produktnummer ABIN7600011

Der Kaninchen Polyklonal Anti-NSMCE2-Antikörper wurde für WB, ELISA, IF, FACS, IP und ICC validiert. Er ist geeignet, NSMCE2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NSMCE2 Antikörper (AA 14-228) (ABIN7600011)

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSMCE2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-NSMCE2 Antikörper

Bindungsspezifität: AA 14-228

Verwendungszweck: Anti-NSMCE2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NSMCE2 Antibody Picoband® (ABIN7600011). Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NSMCE2 recombinant protein (Position: F14-D228).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 12/8/2016. 2. Payne, F., Colnaghi, R., Rocha, N., Seth, A., Harris, J., Carpenter, G., Bottomley, W. E., Wheeler, E., Wong, S., Saudek, V., Savage, D., O'Rahilly, S., Carel, J.-C., Barroso, I., O'Driscoll, M., Semple, R. Hypomorphism in human NSMCE2 linked to primordial dwarfism and insulin resistance. J. Clin. Invest. 124: 4028-4038, 2014. 3. Potts, P. R., Yu, H. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Molec. Cell. Biol. 25: 7021-7032, 2005.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NSMCE2

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Andere Bezeichnung: NSMCE2

Hintergrund:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490

Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Background: This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance.

Molekulargewicht: 34 kDa

Gen-ID: 286053