RNF111 Antikörper (AA 134-994)

Produktnummer ABIN7599978

Der Kaninchen Polyklonal Anti-RNF111-Antikörper wurde für WB, ELISA, IHC, IF, ICC und FACS validiert. Er ist geeignet, RNF111 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für RNF111 Antikörper (AA 134-994) (ABIN7599978)

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF111 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-RNF111 Antikörper

Bindungsspezifität: AA 134-994

Verwendungszweck: Anti-RNF111 Antibody Picoband®

Produktmerkmale: Anti-R Antibody Picoband® (ABIN7599978). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF111 recombinant protein (Position: F134-S994).

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Episkopou, V., Arkell, R., Timmons, P. M., Walsh, J. J., Andrew, R. L., Swan, D. Induction of the mammalian node requires Arkadia function in the extraembryonic lineages. Nature 410: 825-830, 2001. 2. Niederlander, C., Walsh, J. J., Episkopou, V., Jones, C. M. Arkadia enhances nodal-related signalling to induce mesendoderm. Nature 410: 830-834, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu RNF111

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Andere Bezeichnung: RNF111

Hintergrund: E3 ubiquitin-protein ligase Arkadia is an enzyme that in humans is encoded by the RNF111 gene. The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Molekulargewicht: 109 kDa

Gen-ID: 54778