RNF20 Antikörper (AA 123-389)

Produktnummer ABIN7599859

Dieses Anti-RNF20-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RNF20 in WB. Geeignet für Human, Maus und Ratte.

Kurzübersicht für RNF20 Antikörper (AA 123-389) (ABIN7599859)

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RNF20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 3C6E2

Produktdetails anti-RNF20 Antikörper

Bindungsspezifität: AA 123-389

Verwendungszweck: Anti-RNF20 Antibody Picoband® (monoclonal, 3C6E2)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-RNF20 Antibody Picoband® (monoclonal, 3C6E2) (ABIN7599859). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF20 recombinant protein (Position: E123-S389).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
1. Blank, M., Tang, Y., Yamashita, M., Burkett, S. S., Cheng, S. Y., Zhang, Y. E. A tumor suppressor function of Smurf2 associated with controlling chromatin landscape and genome stability through RNF20. Nature Med. 18: 227-234, 2012. 2. Cortez, J. T., Montauti, E., Shifrut, E., Gatchalian, J., Zhang, Y., Shaked, O., Xu, Y., Roth, T. L., Simeonov, D. R., Zhang, Y., Chen, S., Li, Z., and 13 others. CRISPR screen in regulatory T cells reveals modulators of Foxp3. Nature 582: 416-420, 2020. 3. Dickinson, M. E., Flenniken, A. M., Ji, X., Teboul, L., Wong, M. D., White, J. K., Meehan, T. F., Weninger, W. J., Westerberg, H., Adissu, H., Baker, C. N., Bower, L., and 73 others. High-throughput discovery of novel developmental phenotypes. Nature 537: 508-514, 2016. Note: Erratum: Nature 551: 398 only, 2017.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Andere Bezeichnung: RNF20

Hintergrund:

Synonyms: Survival motor neuron protein, Component of gems 1, Gemin-1, SMN1, SMN, SMNT, SMN2, SMNC

Tissue Specificity: Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).

Background: E3 ubiquitin-protein ligase BRE1A is an enzyme that in humans is encoded by the RNF20 gene. The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).

Molekulargewicht: 114 kDa

Gen-ID: 56254