HDC Antikörper (AA 12-662)

Produktnummer ABIN7599827

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HDC in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für HDC Antikörper (AA 12-662) (ABIN7599827)

Target: HDC (Histidine Decarboxylase (HDC))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-HDC Antikörper

Bindungsspezifität: AA 12-662

Verwendungszweck: Anti-Histidine decarboxylase/HDC Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Histidine decarboxylase/HDC Antibody Picoband® (ABIN7599827). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Histidine decarboxylase/HDC recombinant protein (Position: R12-V662).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bruneau, G., Nguyen, V. C., Gros, F., Bernheim, A., Thibault, J. Preparation of a rat brain histidine decarboxylase (HDC) cDNA probe by PCR and assignment of the human HDC gene to chromosome 15. Hum. Genet. 90: 235-238, 1992. 2. Dere, E., De Souza-Silva, M. A., Spieler, R. E., Lin, J. S., Ohtsu, H., Haas, H. L., Huston, J. P. Changes in motoric, exploratory and emotional behaviours and neuronal acetylcholine content and 5-HT turnover in histidine decarboxylase-KO mice.Europ. J. Neurosci. 20: 1051-1058, 2004. 3. Ercan-Sencicek, A. G., Stillman, A. A., Ghosh, A. K., Bilguvar, K., O'Roak, B. J., Mason, C. E., Abbott, T., Gupta, A., King, R. A., Pauls, D. L., Tischfield, J. A., Heiman, G. A., and 16 others. L-histidine decarboxylase and Tourette's syndrome. New Eng. J. Med. 362: 1901-1908, 2010.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu HDC

Target: HDC (Histidine Decarboxylase (HDC))

Andere Bezeichnung: HDC

Hintergrund:

Synonyms: Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43

Tissue Specificity: Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.

Background: HDC(Histidine decarboxylase) is the enzyme that catalyzes the reaction that produces histamine from histidine with the help of vitamin B6. The HDC gene is mapped on 15q21.2. The deduced 662-amino acid protein has a molecular mass of 74,148 Da. The gene contains 12 exons spanning approximately 24 kb. The biogenic amine histamine is an important modulator of numerous physiologic processes, including neurotransmission, gastric acid secretion, and smooth muscle tone. The biosynthesis of histamine from histidine is catalyzed by the enzyme L-histidine decarboxylase. This homodimeric enzyme is a pyridoxal phosphate(PLP)-dependent decarboxylase and is highly specific for its histidine substrate. Ercan-Sencicek et al. noted that animal studies had shown that lack of Hdc in mice results in increased locomotor and stereotypic behaviors, as well as increased anxiety.

Molekulargewicht: 74 kDa

Gen-ID: 3067

UniProt: P19113