NSUN3 Antikörper (AA 12-310)

Produktnummer ABIN7599814

Dieses Anti-NSUN3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NSUN3 in WB, ELISA und FACS. Geeignet für Human und Affe.

Kurzübersicht für NSUN3 Antikörper (AA 12-310) (ABIN7599814)

Target: NSUN3 (NOP2/Sun Domain Family, Member 3 (NSUN3))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NSUN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NSUN3 Antikörper

Bindungsspezifität: AA 12-310

Verwendungszweck: Anti-NSUN3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NSUN3 Antibody Picoband® (ABIN7599814). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NSUN3 recombinant protein (Position: K12-E310).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Haag, S., Sloan, K. E., Ranjan, N., Warda, A. S., Kretschmer, J., Blessing, C., Hubner, B., Seikowski, J., Dennerlein, S., Rehling, P., Rodnina, M., Hobartner, C., Bohnsack, M. T. NSUN3 and ABH1 modify the wobble position of mt-tRNA(Met) to expand codon recognition in mitochondrial translation. EMBO J. 35: 2104-2119, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/25/2017. 3. Nakano, S., Suzuki, T., Kawarada, L., Iwata, H., Asano, K., Suzuki, T. NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met). Nature Chem. Biol. 12: 546-551, 2016.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NSUN3

Target: NSUN3 (NOP2/Sun Domain Family, Member 3 (NSUN3))

Andere Bezeichnung: NSUN3

Hintergrund:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: NSUN3 (NOL1/NOP2/Sun domain family, member 3), also known as MST077, is a 340 amino acid protein that belongs to the methyltransferase superfamily and is thought to function as an S-adenosyl-L-methionine-dependent methyltransferase. The gene encoding NSUN3 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3

Molekulargewicht: 38 kDa

Gen-ID: 63899

UniProt: Q9H649