Anti-GATB Antibody Picoband® (ABIN7599754). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Aufreinigung
Immunogen affinity purified.
Immunogen
E.coli-derived human GATB recombinant protein (Position: R113-E443). Human GATB shares 89.7% amino acid (aa) sequence identity with mouse GATB.
PET112
Reaktivität: Maus
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 680
Applikationshinweise
Western blot, 0.25-0.5 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Friederich, M. W., Timal, S., Powell, C. A., Dallabona, C., Kurolap, A., Palacios-Zambrano, S., Bratkovic, D., Derks, T. G. J., Bick, D., Bouman, K., Chatfield, K. C., Damouny-Naoum, N., and 41 others. Pathogenic variants in glutamyl-tRNA(Gln) amidotransferase subunits cause a lethal mitochondrial cardiomyopathy disorder. Nature Commun. 9: 4065, 2018. Note: Electronic Article. 2. Gross, M. B. Personal Communication. Baltimore, Md. 11/30/2016. 3. Nagao, A., Suzuki, T., Katoh, T., Sakaguchi, Y., Suzuki, T. Biogenesis of glutaminyl-mt tRNA(Gln) in human mitochondria. Proc. Nat. Acad. Sci. 106: 16209-16214, 2009.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Target
PET112L (PET112)
(PET112 Homolog (PET112))
Andere Bezeichnung
GATB
Hintergrund
GATB allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln).