L3MBTL3 Antikörper (AA 11-686)

Produktnummer ABIN7599730

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch L3MBTL3 in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für L3MBTL3 Antikörper (AA 11-686) (ABIN7599730)

Target: L3MBTL3 (L(3)mbt-Like 3 (L3MBTL3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser L3MBTL3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-L3MBTL3 Antikörper

Bindungsspezifität: AA 11-686

Verwendungszweck: Anti-L3MBTL3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-L3MBTL3 Antibody Picoband® (ABIN7599730). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human L3MBTL3 recombinant protein (Position: Q11-R686).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Arai, S., Miyazaki, T. Impaired maturation of myeloid progenitors in mice lacking novel Polycomb group protein MBT-1. EMBO J. 24: 1863-1873, 2005. 2. Xu, T., Park, S.-S., Giaimo, B. D., Hall, D., Ferrante, F., Ho, D. M., Hori, K., Anhezini, L., Ertl, I., Bartkuhn, M., Zhang, H., Milon, E., and 15 others. RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression of Notch signaling via histone demethylase KDM1A/LSD1. EMBO J. 36: 3232-3249, 2017. 3. Zhang, C., Leng, F., Saxena, L., Hoang, N., Yu, J., Alejo, S., Lee, L., Qi, D., Lu, F., Sun, H., Zhang, H. Proteolysis of methylated SOX2 protein is regulated by L3MBTL3 and CRL4-DCAF5 ubiquitin ligase. J. Biol. Chem. 294: 476-489, 2019.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu L3MBTL3

Target: L3MBTL3 (L(3)mbt-Like 3 (L3MBTL3))

Andere Bezeichnung: L3MBTL3

Hintergrund:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.

Molekulargewicht: 90 kDa

Gen-ID: 84456