EIF5 Antikörper (AA 11-423)

Produktnummer ABIN7599722

Der Kaninchen Polyklonal Anti-EIF5-Antikörper wurde für WB, ELISA, IHC und FACS validiert. Er ist geeignet, EIF5 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für EIF5 Antikörper (AA 11-423) (ABIN7599722)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-EIF5 Antikörper

Bindungsspezifität: AA 11-423

Verwendungszweck: Anti-EIF5 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EIF5 Antibody Picoband® (ABIN7599722). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF5 recombinant protein (Position: D11-D423).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Das, S., Ghosh, R., Maitra, U. Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J. Biol. Chem. 276: 6720-6726, 2001. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/5/2010. 3. Jennings, M. D., Pavitt, G. D. eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature 465: 378-381, 2010. Note: Erratum: Nature 468: 122 only, 2010.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Andere Bezeichnung: EIF5

Hintergrund:

Synonyms: Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.

Molekulargewicht: 49 kDa

Gen-ID: 1983

UniProt: P55010