EIF5 Antikörper (AA 11-423)

Produktnummer ABIN7599721

Dieses Maus Monoklonal-Antikörper erkennt spezifisch EIF5 in WB, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EIF5 Antikörper (AA 11-423) (ABIN7599721)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 2F13C4

Produktdetails anti-EIF5 Antikörper

Bindungsspezifität: AA 11-423

Verwendungszweck: Anti-EIF5 Antibody Picoband® (monoclonal, 2F13C4)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EIF5 Antibody Picoband® (monoclonal, 2F13C4) (ABIN7599721). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF5 recombinant protein (Position: D11-D423).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Das, S., Ghosh, R., Maitra, U. Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J. Biol. Chem. 276: 6720-6726, 2001. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/5/2010. 3. Jennings, M. D., Pavitt, G. D. eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature 465: 378-381, 2010. Note: Erratum: Nature 468: 122 only, 2010.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Andere Bezeichnung: EIF5

Hintergrund:

Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.

Molekulargewicht: 49 kDa

Gen-ID: 1983

UniProt: P55010